Olga Igorevna Filippova
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The degradation of glyphosate and its effect on microbial community of agrosod-podzolic soil under the short-term model experience conditionsMoscow University Bulletin. Series 17. Soil science. 2020. N 3. p.47-55read more1476
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In laboratory conditions, the decomposition of glyphosate with the formation of aminomethylphosphonic acid (AMPA) and its effect on the total abundance of bacteria and fungi, as well as the number of copies of genes encoding the enzymes C-P lyase of a-proteobacteria ( phnJ ), acid and alkaline phosphatase ( phoC and phoD ) and Fe-protein nitrogenase ( nifH ). It is shown that when applying glyphosate in recommended doses (5-10 mg/kg) 5-7% of the introduced herbicide is detected after 14 days, but when the dose is increased to 100 mg/kg, this value increases to 23%. Slowing down the rate of degradation of the herbicide appears only in the first week of incubation and is accompanied by a decrease in the number of copies of the phoC, phoD, nifH genes and an increase in the abundance of fungi. The obtained results indicate a predominant degradation of glyphosate with the C-P bond breaking and the formation of phosphates, as well as a possible inhibition of the nitrogen fixation process. It is shown that at an application dose of glyphosate 100 mg/kg, the accumulation of the first metabolite AMPA can occur during the degradation of the herbicide with a break in the C—N bond. Bioassay using wheat showed that when applying glyphosate at a dose of 100 mg/kg, there is an inhibition of plant development: the length of the roots and the biomass of the shoots reduced by 60 and 20% compared to the control, respectively. Based on the obtained data, it is proposed to use the reduction in the content of copies of the phoC gene and the growth of copies of ITS rRNA as indicators of the predominant decomposition of glyphosate through the sarcosin pathway. A reduction of copies of ITS rRNA gene by 40% or more can be used as an indicator of the possibility of AMPA accumulation during glyphosate degradation.
Keywords: amino-methyl-phosphonic acid; polymerase chain reaction; phoC; phoD; nifH
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An assessment of the most important carbon pools in the mixed forests of the Moscow regionMoscow University Bulletin. Series 17. Soil science. 2025. N 3. p.30-41Alexey S. Sorokin Valeria M. Telesnina M. A. Podvezennaya Yulia. L. Meshalkina Olga Iv. Manakova Vera P. Samsonova Marina I. Kondrashkina Svetlana Ev. Dyadkina Mikhail R. Chekin Igor A. Ilyichev Svetlana. A. Kulachkova Olga I. Filippovaread more743
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The carbon reserves in main components of Moscow region coniferous-broadleaf forest were studied: various fractions of the tree stand, dead wood, mortmass, litter, living ground cover and mineral profile of the soil. To assess the potential intensity of organic matter decomposition, a number of indicators of the functioning of the microbial biomass were determined. An assessment is given of the carbon reserves and their shares in the ecosystem components that differ in the rate of renewal and potential capacity for carbon sequestration, as well as the degree of their spatial variation. The total carbon pool of the studied forest ecosystem is 18.7+0.8 kg·m–2, with almost 90% of the total stock concentrated in the perennial parts of the tree stand, dead wood, mortmass and mineral profile of the soil. These most stable pools are characterized by the least spatial variation within the biogeocenosis. The carbon reserves of the assimilating part of the grass layer and tree leaves are only 0.02 and 0.08 kg·m–2, respectively. The carbon reserves of litter are quite low - 0.21 ± 0.04 kg·m–2, which does not exceed 2% of the total carbon reserves of the ecosystem. The data obtained indicate that even secondary subclimax forest ecosystems are a significant absorber of atmospheric carbon, mainly due to the mass of the tree stand and soil organic matter.Keywords: secondary birch-spruce forest; soil organic matter; biological cycle; EE SEC "Chashnikovo"; carbon reserve; carbon sink
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The effect of humic preparations on crop yields and quality: a systematic review and meta-analysisMoscow University Bulletin. Series 17. Soil science. 2026. N 2. p.27-43read more71
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The study aimed to perform a systematic study and meta-analysis of national and foreign data (687 data points in total) on the effect of humic preparations (HPs) on crop yields. Specific objectives included evaluating the efficiency range of HPs (yield increase as a percentage of the control) accounting for statistical uncertainty estimated via absolute median deviation, and assessing the influence of HPs characteristics, crop types, and soil properties on this metric. HPs efficiency varied from –59% to +540% (mean 18%) and followed a non-normal distribution. The median efficiency was 12 %, with lower and upper quartiles at 5% and 21%, respectively, and uncertainty of 99 %. Kruskal–Wallis test revealed that soil granulometric composition and HPs enrichment with trace elements did not significantly affect efficiency of HPs. Key influencing factors included: cation salts of humate in HPs (Na salts were the least effective); HPs application method (combined soil and foliar application was most effective); soil acidity and humus content (higher efficiency at pH > 6.6 and humus content < 1%). Plants in the aster family showed the greatest responsiveness to HPs, while legumes and nightshades exhibited significantly lower efficiency. Under nitrogen deficiency, HPs efficiency rose to 22%, and most studies reported increased nitrogen and/or protein content in crop products. These findings suggest that HPs primarily enhance field yields by improving plant nitrogen assimilation. The results indicate that promising future research should evaluate HPs effectiveness against reduced nitrogen fertilizer rates. Given rising fertilizer costs, even modest effects could prove economically viable. Further field studies should optimize HPs use conditions and methods, prioritizing identification of factors driving efficiency uncertainty — preliminarily, application method and soil conditions (pH, humus content, and available nitrogen).Keywords: humic substances; humates; field experiments; nitrogen deficiency; Asteraceae; nitrate transporters NRT; Classification and Regression Trees; uncertainty; economic efficiency
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