Shulakov Andrey Yuryevich

Analysis of tilosin migration in sand and sod-podzolic soil: a laboratory filtration experiment A. K. Toskhoporan, A. P. Kiryushina, S. V. Patsaeva, A. Yu. Shulakov, A. D. Batakov, V. A. Terekhova, A. B. Umarova The migration of the antibiotic tylosin in fine-grained quartz sand and the upper layer of sodpodzolic soil was studied in laboratory filtration experiments to obtain output curves and calculate the parameters of antibiotic migration in variants with different pore structure. It was found that the transfer of tylosin can be tracked by analyzing its concentration in filtrate portions, measured by the absorption spectra of the antibiotic. It has been experimentally shown that the appearance of an antibiotic occurs in the first shift of the entire pore space of the column. Tylosin actively migrates in all variants of sod-podzolic soil, and its erosion front reaches 40 cm. Similar behavior of antibiotics and potassium cations in soil was revealed. A high negative correlation (k = 79% according to Pearson) was found between the direct method of determining the tylosin content in filtrates by spectrophotometry and its algotoxicity in migration experiments. Keywords:soil, porosity, granulometric composition, output curves, macrolide antibiotic, mixing step, migration, spectrophotometry, ecotoxicity.