Analysis of tilosin migration in sand and sod-podzolic soil: a laboratory filtration experiment
Abstract
Analysis of tilosin migration in sand and sod-podzolic soil: a laboratory filtration experiment A. K. Toskhoporan, A. P. Kiryushina, S. V. Patsaeva, A. Yu. Shulakov, A. D. Batakov, V. A. Terekhova, A. B. Umarova The migration of the antibiotic tylosin in fine-grained quartz sand and the upper layer of sodpodzolic soil was studied in laboratory filtration experiments to obtain output curves and calculate the parameters of antibiotic migration in variants with different pore structure. It was found that the transfer of tylosin can be tracked by analyzing its concentration in filtrate portions, measured by the absorption spectra of the antibiotic. It has been experimentally shown that the appearance of an antibiotic occurs in the first shift of the entire pore space of the column. Tylosin actively migrates in all variants of sod-podzolic soil, and its erosion front reaches 40 cm. Similar behavior of antibiotics and potassium cations in soil was revealed. A high negative correlation (k = 79% according to Pearson) was found between the direct method of determining the tylosin content in filtrates by spectrophotometry and its algotoxicity in migration experiments. Keywords:soil, porosity, granulometric composition, output curves, macrolide antibiotic, mixing step, migration, spectrophotometry, ecotoxicity.References
1. Антропова Н.С., Ушакова О.В., Водянова М.А. и др. Риск распространения антибиотикорезистентности через объекты окружающей среды и продукты питания (обзор) // Российский журнал восстановительной медицины. 2020. № 4. С. 36–51. 2. Корсунская Л.П. Гидродинамические и физические свойства почв: Автореф. дис. … канд. биол. наук. М., 1997. 18 с. 3. Парамонов С.Г., Зеликова Д.Д., Склярова Л.В. и др. Экологические риски при микрозагрязнениях тетрациклином окружающей среды // Формулы Фармации. 2022. Т. 4, № 1. С. 76–88. https://doi.org/10.17816/phf106547 4. Терехова В.А., Батаков А.Д., Кирюшина А.П. и др. Биотестирование и спектрофотометрия для нормирования ветеринарных антибиотиков в почвах и сопредельных средах // Экология и промышленность России. 2025. Т. 29, № 8. С. 30–37. 5. Умарова А.Б. Преимущественные потоки влаги в почвах: закономерности формирования и значение в функционировании почв. М., 2011. 269 с. 6. Умарова А.Б., Бутылкина М.А., Гасина А.И. и др. Трансформация водоудерживающей и транспортной функций искусственных почв в условиях г. Москвы // Вестн. Моск. ун-та. Сер. 17. Почвоведение. 2024. Т. 79, № 4. С. 14–25. https://doi.org/10.55959/MSU0137-0944-17-2024-79-4-14-25 7. Шеин Е.В., Щеглов Д.И., Умарова А.Б. и др. Структурное состояние техноземов и формирование в них преимущественных потоков влаги // Почвоведение. 2009. № 6. С. 687– 695. 8. Щеглов Д.И., Брехова Л.И. Процессы почвообразования. Воронеж, 2016. 58 с. 9. Aga D.S., O’Connor S., Ensley S. et al. Determination of the persistence of tetracycline antibiotics and their degradates in manure-amended soil using enzyme-linked immunosorbent assay and liquid chromatography-mass spectrometry // Journal of Agricultural and Food Chemistry. 2005. Vol. 53. P. 7165–7171. 15. Ardakani Z., Aragrande M., Canali M. Global antimicrobial use in livestock farming: an estimate for cattle, chickens, and pigs // Animal. 2024. Vol. 18, № 2. Art. 101060. https://doi.org/10.1016/j.animal.2023.101060 16. Blackwell P.A., Kay P., Ashauer R. et al. Effects of agricultural conditions on the leaching behaviour of veterinary antibiotics in soils // Chemosphere. 2009. Vol. 75, № 1. P. 13–19. https://doi.org/10.1016/j.chemosphere.2008.11.070 17. Chessa L., Pusino A., Garau G. et al. Soil microbial response to tetracycline in two different soils amended with cow manure // Environmental Science and Pollution Research. 2016. Vol. 23. P. 5807–5817. https://doi.org/10.1007/s11356-015-5966-8 18. Chen X., Song Y., Ling C. et al. Fate of emerging antibiotics in soil-plant systems: a case on fluoroquinolones // Science of The Total Environment. 2024. Vol. 951. Art. 175487. https://doi.org/10.1016/j.scitotenv.2024.175487 19. Christian T., Schneider R.J., Färber H.A. et al. Determination of antibiotic residues in manure, soil, and surface waters // Acta Hydrochimica et Hydrobiologica. 2003. Vol. 31, № 1. P. 36–44. https://doi.org/10.1002/aheh.200390014 20. Deroco P.B., Rocha-Filho R.C., Fatibello-Filho O. A new and simple method for the simultaneous determination of amoxicillin and nimesulide using carbon black within a dihexadecylphosphate film as electrochemical sensor // Talanta. 2018. Vol. 179. P. 115–123. https://doi.org/10.1016/j.talanta.2017.10.048 21. Franco A., Trapp S. Estimation of the soil–water partition coefficient normalized to organic carbon for ionizable organic chemicals // Environmental Toxicology and Chemistry. 2008. Vol. 27, № 10. P. 1995–2004. https://doi.org/10.1897/07-583.1 22. Franco A., Fu W., Trapp S. Influence of soil pH on the sorption of ionizable chemicals: modeling advances // Environmental Toxicology and Chemistry. 2009. Vol. 28, № 3. P. 458–464. 23. Jones A.D., Bruland G.L., Agrawal S.G., et al. Factors influencing the sorption of oxytetracycline to soils // Environmental Toxicology and Chemistry. 2005. Vol. 24. P. 761–770. https://doi.org/10.1897/04-125R1.1 24. Kim S.-C., Carlson K. Temporal and spatial trends in the occurrence of human and veterinary antibiotics in aqueous and river sediment matrices // Environmental Science & Technology. 2007. Vol. 41, № 1. P. 50–57. 25. Kolz A.C., Ong S.K., Moorman T.B. Sorption of tylosin onto swine manure // Chemosphere. 2005. Vol. 60, № 2. P. 284–289. 26. Lin L., Hu X., Liang J. et al. Adsorption of tylosin in wastewater by iron-rich farmland soil and the effect of iron reduction and common cations // Water Reuse.2021. Vol. 11, № 2. P. 248– 256. 27. Li Y., Li Q., Ji Z. et al. Current status and spatiotemporal evolution of antibiotic residues in livestock and poultry manure in China // Agriculture. 2023. Vol. 13, № 10. Art. 1877. https://doi.org/10.3390/agriculture13101877 28. McCoy L.S., Xie Y., Tor Y. Antibiotics that target protein synthesis // Wiley Interdisciplinary Reviews: RNA. 2011. Vol. 2, № 2. P. 209–232. https://doi.org/10.1002/wrna.60 29. Rasheela A.R.P., Khalid M.F., Abumaali D.A. et al. Impact of abiotic stressors on soil microbial communities: a focus on antibiotics and their interactions with emerging pollutants // Soil Systems. 2025. Vol. 9, № 1. Art. 2. https://doi.org/10.3390/soilsystems9010002 30. Salam A.H. Al-Ameri, Al-Waeli N.M.H. Differential pulse polarographic study of amoxicillin and ciprofloxacin and its determination in pharmaceuticals // International Journal of Bioanalytical Methods and Bioequivalence Studies. 2016. Vol. 3, № 1. P. 47–54. https://doi.org/10.19070/2470-4490-150006 31. Schlüsener M.P., Bester K., Spiteller M. Determination of antibiotics such as macrolides, ionophores and tiamulin in liquid manure by HPLC-MS/MS // Analytical and Bioanalytical Chemistry. 2003. Vol. 375, № 7. P. 942–947. 32. Tolls J. Sorption of veterinary pharmaceuticals in soils: a review // Environmental Science & Technology. 2001. Vol. 35, № 17. P. 3397–3406. https://doi.org/10.1021/es0003021 33. Wollenberger L., Halling-Sørensen B., Kusk K.O. Acute and chronic toxicity of veterinary antibiotics to Daphnia magna // Chemosphere. 2000. Vol. 40, № 7. P. 723–730. 34. Wohde M., Berkner S., Junker T. et al. Occurrence and transformation of veterinary pharmaceuticals and biocides in manure: a literature review // Environmental Sciences Europe. 2016. Vol. 28. P. 1–25. 35. Yuan Q., Sui M., Qin C. et al. Migration, transformation and removal of macrolide antibiotics in the environment: a review // Environmental Science and Pollution Research International. 2022. Vol. 29, № 18. P. 26045–26062. https://doi.org/10.1007/s11356-021-18251-2 36. Zhang Q. et al. Sorption of tylosin on agricultural soils // Soil Science. 2011. Vol. 176, № 8 P. 407–412. https://doi.org/10.1097/SS.0b013e3182247420 37. Zhang Q., Peng Q., Shu X. et al. Spectroscopic analysis of tylosin adsorption on extracellular DNA reveals its interaction mechanism // Colloids and Surfaces B: Biointerfaces. 2019. Vol. 183. Art. 110431. https://doi.org/10.1016/j.colsurfb.2019.110431 38. Zhang Y. et al. Vertical migration of antibiotics during rainfall throughout a year in longterm manure-fertilized soils differing in pH // Journal of Hazardous Materials. 2025. Vol. 494. Art. 138578. https://doi.org/10.1016/j.jhazmat.2025.138578
PDF, ru

This work is licensed under a Сreative Commons Atribiution - NonCommercial 4.0 International (CC BY-NC 4.0)
Accepted date: 08/30/2026
Keywords: soil; porosity; granulometric composition; output curves; macrolide antibiotic; mixing step; migration; spectrophotometry; ecotoxicity
DOI: 10.55959/MSU0137-0944-17-2026-81-3-231-242
Available in the on-line version with: 29.08.2026
-
To cite this article:

This work is licensed under a Сreative Commons Atribiution - NonCommercial 4.0 International (CC BY-NC 4.0)

