Taxonomic structure of the bacterial complex in ant-infested fallow soils, colonized by LASIUS NIGER AND LASIUS FLAVUS ANTS

Abstract

We obtained data on the number of saprotrophic bacteria and the structure of bacterial complexes of cultivated bacteria of the soil of the fallow field, the material of anthills located in the field under study, and ants of the species Lasius niger and Lasius flavus, which are dominant species in this fallow field. It has been established that ants of two closely related species from the genus Lasius differ significantly in the number and composition of the cultivated bacteria associated with them, and have a different effect on the microbiocenosis of the soils they inhabit. A high similarity of bacterial complexes of L.niger ants and the material of their nests was shown, at the same time, such a pattern was not revealed for L. flavus. It has been established that the structural components of anthills differ in the number and composition of cultivated bacteria. It has been shown that in general, anthills are loci of increased abundance and higher bacterial diversity compared to control soils, which can be traced not only in the surface soil layer. It has been found that the nesting activity of ants promotes the redistribution of microorganisms down the profile, as a result of which the increased biodiversity of microorganisms is maintained at a greater depth than in the control. To a greater extent, this phenomenon is characteristic of soil-dwelling ants L. flavus. In anthills, an increased number of fertility-improving bacteria with high biotechnological potential, such as Pseudomonas koreensis, capable of fixing atmospheric nitrogen, and Paenibacilluschitinolyticus, showing chitinolytic activity, was found compared to control. According to the results of additional studies, representatives of Agrobacterium radiobacter, not identified by direct inoculation, were identified from the accumulation culture on Ashby's nutrient medium. With the help of genome-wide sequencing, several species of bacteria were identified that were not determined by mass spectrometry.

References

1. Галынкин В.А., Заикина Н.А., Кочеровец В.И. и др. Питательные среды для микробиологического контроля качества лекарственных средств и пищевых продуктов: Справочник. М., 2024. 336 с. 2. Голиченков М.В., Нейматов А.Л., Кирюшин А.В. Микробиологическая активность почв, заселенных муравьями Lasiusniger // Почвоведение. 2009. № 7. С. 847–852. 3. Дайнеко Д.В., Русаков А.В. Влияние муравейников на залежные почвы (на примере Ярославской и Ленинградской областей) // Вестн. СПбГУ. 2012. Сер. 3. Вып. 2. С. 120–130. 4. Егоров В.В.,Фридланд В.М., Иванова Е.Н. и др. Классификация и диагностика почв СССР. М., 1977. 264 с. 5. Закалюкина Ю.В., Бирюков М.В., Нетрусов А.И.Фенотипическая и филогенетическая характеристика актиномицетов, выделенных из муравьев LasiusnigerиFormicacunicularia // Вестн.Моск. ун-та. Сер. 16. Биология. 2017. Т. 72, № 1. С. 16–23. 6. Зрянин В.А. Влияние муравьев рода Lasius на почвы луговых биогеоценозов // Успехи современной биологии. 2003. Т. 123, № 3. С. 278–288. 7. Кокорева А.А., Голиченков М.В., Приболовец А.И. и др.Физические свойства слаборазвитых постагрогенных песчаных почв под муравьиными гнездами (Рязанская область) // Всероссийский мирмекологический симпозиум (Москва, 27–31 августа 2022 года): материалы. 2022. С. 82–87. 8. Кондрашов Б.С., Седых С.Е. Способ скрининг биохимических активностей почвенных бактерий с использованием специфических сред и метода ПЦР // Школа-конференция молодых ученых, аспирантов и студентов «Генетические технологии в микробиологии и микробное разнообразие». 2025. C. 29. https://doi.org/10.24412/cl-37442-2025-1-029-030 9. Котова А.А., Голиченков М.В., Умаров М.М. и др. Микробиологическая активность в муравейниках залежных земель (Рязанская область) // Вестн. Моск. ун-та. Сер. 17. Почвоведение. 2013. № 2. С. 31–34. 10. Котова А.А., Умаров М.М., Закалюкина Ю.В. Особенности трансформации азота и углерода в гнездах почвообитающих муравьев // Вестн. Моск. ун-та. Сер. 17. Почвоведение. 2015. № 1. С. 30–34. 11. Полевой определитель почв России. 2008. М., 2008. 182 с. 12. Потехина Н.В., Шашков А.С., Тульская Е.М. и др.Галактофуранан клеточной стенки актинобактерий рода Paenarthrobacter // Микробиология. 2021. T. 90, № 1. С. 122–128. 13. Турабаева Г.К., Оспанова Г.С., Бозшатаева Г.Т. Результаты изучения муравьев в качестве биоиндикаторов почвы // Международный журнал прикладных и фундаментальных исследований. 2016. № 2–1. С. 44–47. 14. Чурилина А.Е., Голиченков М.В., Иванова А.Е.и др. Биологическая активность муравьиных гнезд Среднетаежной зоны // Вестн. Моск. ун-та. Сер. 17. Почвоведение. 2017. № 4. С. 40–44. 15. Baby G., Shea P., Sudhakar D. et al. Potential use of Pseudomonas koreensis AGB-1 in association with Miscanthus sinensis to remediate heavy metal(loid)-contaminated mining site soil // Journal of Environmental Management. 2015. № 151. Р.160–166. https://doi.org/10.1016/j.jenvman.2014.12.045 16. Busse H.J. Review of the taxonomy of the genus Arthrobacter, emendation of the genus Arthrobacter sensu lato, proposal to reclassify selected species of the genus Arthrobacter in the novel genera Glutamicibacter gen. nov., Paeniglutamicibacter gen. nov., Pseudoglutamicibacter gen. nov., Paenarthrobacter gen. nov. and Pseudarthrobacter gen. nov., and emended description of Arthrobacter roseus // Int. J. Syst. Evol. Microbiol. 2016. Vol. 66. P. 9–37. https://doi.org/10.1099/ijsem.0.000702 17. Czechowski W., Radchenko A., Czechowska W. The ants (Hymenoptera, Formicidae) of Poland // Museum and Institute of Zoology, Polish Academy of Sciences. Warsaw. 2002. P.200. 18. Dymova A., Kovalev M., Silantyev A. et al. Unusual genomic and biochemical features of Paenarthrobacterlasiusi sp. nov-a novel bacterial species isolated from Lasius niger anthill soil // International Journal of Molecular Sciences. 2025. Vol. 26, № 1.P. 67. https://doi.org/10.3390/ijms26010067 19. Golichenkov M.V., Maksimova I.A., Zakalyukina Yu V. et al. Ants’ nesting activity as a factor of changes in soil physical properties // IOP Conf. Series: Earth and Environmental Science. 2019. Vol. 368. P. 1–5. https://doi.org/10.1088/1755-1315/368/1/012013 20. Khan Z., Kim S.G., Jeon Y.H. et al. A plant growth promoting rhizobacterium, Paenibacilluspolymyxa strain GBR-1, suppresses root-knot nematode // Bioresource Technology. 2008. Vol. 99. № 8. Р. 3016–3023.https://doi.org/10.1016/j.biortech.2007.06.031 21. Kieft T.L. New allometric scaling laws revealed for microorganisms // Trends in Ecol. Evolut. 2017. Vol. 32, № 6, P.400-402. https://doi.org/10.1016/j.tree.2017.02.017 22. Larkin M.A., Blackshields G., Brown N.P.et al.Clustal W and Clustal X version 2.0 // Bioinformatics. 2007. Vol. 23. P. 2947–2948. 23. Macleod K., Rumbold K., Padayachee K.A. Systems Approach to Uncover the Effects of the PGPR Pseudomonas koreensison the Level of Drought Stress Tolerance in Helianthus Annuus // Procedia Environmental Sciences. 2015. Vol. 29. P. 262–263. https://doi.org/10.1016/j.proenv.2015.07.200 24. Mikhalenko B., Kirpotin S.N., Babenko A.S. Development and transformation of floodplain territories by ants // Acta Biologica Sibirica. 2023. Vol. 9. P. 943–952. https://doi: 10.5281/zenodo.10101342 25. Parte A., Carbasse J., Meier-Kolthoff J.et al. List of Prokaryotic names with Standing in Nomenclature – integrated into the DSMZ’s Prokaryotic Nomenclature Up-to-date (PNU) database // Int. J. Syst. Evol.Microbiol. 2020. Vol. 70(11). P.5607–5612. https://doi.org/10.1099/ijsem.0.004332 26. Pinto-Tomás A.A., Anderson M.A., Suen G.et al. Symbiotic Nitrogen Fixation in the Fungus Gardens of Leaf-Cutter Ants // Science. 2009. P. 1120-1123. https://doi.org/10.1126/science.1173036 27. Rodriguez R.L., Gunturu S., Harvey W.T. et al. The Microbial Genomes Atlas (MiGA) webserver: taxonomic and gene diversity analysis of Archaea and Bacteria at whole genome level // Nucleic Acids Res. 2018. Vol. 46. P. 282–288. https://doi.org/10.1093/nar/gky467 28. Songa Y., Seoa D., Kima K.et al. Expression patterns of chitinase produced from Paenibacilluschitinolyticus with different culture media // Carbohydrate Polymers. 2012. Vol. 90,Iss. 2. P. 1187-1192. https://doi.org/10.1016/j.carbpol.2012.06.016 29. Wilson E.O., Hoelldobler B. et al.The rise of the ants: A phylogenetic and ecological explanation // Proceedings of the National Academy of Sciences of the United States of America. 2005. № 102(21). Р. 7411–7414. https://doi.org/10.1073/pnas.0502264102