The role of foliar application of aminoacids on the development of barley in conditions of soil contamination with cadmium

Abstract

The article considers the possibility of foliar application of amino acids (proline, methionine and lysine) for the regulation of metabolism and the formation of stress resistance in plants contaminated with heavy metals (cadmium). In the green pot experiment, stress was created due to the introduction of Cd(NO3)2 into the soil at a dose of 10 mg·kg–1, which corresponds to 5 permissible concentration (SanPiN 1.2.3685-21). In an experiment with «Nur» barley, the effect of these amino acids on biomass, the content of total and protein nitrogen in leaves, the content of pigments and their ratios (Cl a, Cl b, carotenoids, Cl a / Cl b) were studied. Foliar treatment of plants with amino acids in the absence of stressful conditions was accompanied by a significant increase in aboveground biomass. The use of proline, methionine and lysine in conditions of high Cd concentrations in the soil had a positive effect on barley biomass and ear formation. All plants treated with proline and lysine showed an increase in the content of all pigments (Cl a, Cl b, carotenoids) compared with the control. Methionine had a positive effect on the chlorophyll content precisely under stress, whereas in the absence of stress, the pigment content in the plant decreased. In the Cd-contaminated variants, there was a decrease in the content of both total and protein nitrogen in barley, which indicated a violation of metabolic processes in the plant under stress. Foliar treatment with amino acids during the tillering phase is accompanied by an increase in nitrogen content both under stress and without it.

References

1. Афонина Т.Е. Анализ качественного состояния сельскохозяйственных земель в Иркутской области // Климат, экология, сельское хозяйство Евразии: Материалы XI Международной научно-практической конференции (28–29 апреля 2022 г.). Иркутск, 2022. 2. Битюцкий Н.П. Микроэлементы высших растений. СПб., 2020. 3. Временный максимально допустимый уровень (МДУ) содержания некоторых химических элементов и госсипола в кормах для сельскохозяйственных животных и кормовых добавках (утв. Главным управлением ветеринарии Государственного агропромышленного комитета СССР 7 августа 1987 г.). 4. Методы биохимического анализа растений / Под ред. В.В. Полевого, Г.Б. Максимова. Л., 1978. 5. Пастухова Н.Л. Детоксикация тяжелых металлов у растений // Проблемы экологии и охраны природы техногенного региона: Межведомственный сборник научных работ / Отв. ред. С.В. Беспалова. Донецк, 2008. 6. Потатуева Ю.А., Прищеп Е.Г., Сидоренкова Н.К. и др. Влияние карбоната кадмия на урожай сельскохозяйственных культур, подвижность кадмия в почве и накопление растениями // Агрохимия. 2005. № 8. 7. Практикум по агрохимии / Под ред. В.Г. Минеева. М., 1989. 8. Рассохина Е.В., Векшин М.Ю., Дягелев А.А. Почвенная утилизация осадка сточных вод // Химия. Экология. Урбанистика. 2023. Т. 1. 9. Титов А.Ф., Казнина Н.М., Таланова В.В. Тяжелые металлы и растения. Петрозаводск, 2014. 10. Узаков З.З. Тяжелые металлы и их влияние на растения // Символ науки. 2018. № 1–2. 11. Ульяненко Л.Н., Филипас А.С., Лой Н.Н. и др. Влияние загрязнения кадмием дерново-подзолистой почвы на рост и развитие растений ячменя // Агрохимия. 2009. № 6. 12. Abdelkader M., Voronina L., Puchkov M. et al. Seed Priming with Exogenous Amino Acids Improves Germination Rates and Enhances Photosynthetic Pigments of Onion Seedlings (Allium cepa L.) // Horticulturae. 2023. Vol. 9, № 80. 13. Abdelkader M., Suliman A.A., Salem S.S. et al. Studying the Combined Impact of Salinity and Drought Stress-Simulated Conditions on Physio-Biochemical Characteristics of Lettuce Plant // Horticulturae. 2024. Vol. 10, № 11. 14. Alfosea-Simón M., Zavala-Gonzalez E.A., Camara-Zapata J.M. et al. Effect of foliar application of amino acids on the salinity tolerance of tomato plants cultivated under hydroponic system // Scientia Horticulturae. 2020. Vol. 272. 15. Almas, H.I., -un-Nisa, Z., Anwar, S. et al. Exogenous application of methionine and phenylalanine confers salinity tolerance in tomato by concerted regulation of metabolites and antioxidants // Journal of Soil Science and Plant Nutrition. 2021. Vol. 21, № 4. 16. Aragón W., Reina-Pinto J.J, Serrano M. The intimate talk between plants and microorganisms at the leaf surface // J. Exp. Bot. 2017. Vol. 68, № 19. 17. Ashouri R., Fallah H., Niknezhad Y. et al. Effect of application of plant growth promoting bacteria and amino acids foliar application on growth characteristics, yield, and nutritional value of rice (Oryza sativa L.) // Iranian Journal of Field Crops Research. 2023. Vol. 21, № 3. 18. Aydemir Ö., Korkmaz K., Özkutlu F. The effect of foliar application of different amino acids (L-histidine, methionine) on cadmium and zinc uptake of wheat // Turkish Journal of Agriculture-Food Science and Technology. 2022. Vol. 10, № 8. 19. Bakhoum G.S., Badr E.A.E., Sadak M.S. et al. Improving Growth, Some Biochemical Aspects and Yield of Three Cultivars of Soybean Plant by Methionine Treatment Under Sandy Soil Condition // Int. J. Environ. Res. 2019. № 13. 20. Baqir H.A., Zeboon,N.H., Al-Behadili A.A.J. The role and importance of amino acids within plants: A review // Plant Archives. 2019. Vol. 19(2). 21. Chen X., Tao H., Wu Y. et al. Effects of Cadmium on metabolism of photosynthetic pigment and photosynthetic system in Lactuca sativa L. revealed by physiological and proteomics analysis // Scientia Horticulturae. 2022. Vol. 305. 22. Colovic M.B., Vasic V.M., Djuric D.M. et al. Sulphur-containing amino acids: protective role against free radicals and heavy metals // Current medicinal chemistry. 2018. Vol. 25, № 3. 23. EL-Bassiouny H.M.S. Physiological responses of wheat to salinity alleviation by nicotinamide and tryptophan // Int. J. Agric. Biol. 2005. Vol. 7, № 4. 24. Forde B.G., Roberts M.R. Glutamate receptor-like channels in plants: a role as amino acid sensors in plant defence? // F1000prime Reports. 2014. Vol. 6. 25. Galili G., Tang G., Zhu X., Gakiere B. Lysine catabolism: a stress and development super-regulated metabolic pathway // Current opinion in plant biology. 2001. Vol. 4(3). 26. Jeber B., Khaeim H. Effect of foliar application of amino acids, organic acids, and naphthalene acetic acid on growth and yield traits of wheat // Plant Аrchives. 2019. Vol. 19, № 2. 27. Kishor P., Sangam S., Amrutha R.N. et al. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance // Current Science. 2005. Vol. 88, № 3. 28. Lu Y., Wang Qf., Li J. et al. Effects of exogenous sulfur on alleviating cadmium stress in tartary buckwheat // Sci. Rep. 2019. № 9. 29. Ma C., Wang Y., Gu D. et al. Overexpression of S- adenosyl-L-methionine synthetase 2 from sugar beet M14 increased Arabidopsis tolerance to salt and oxidative stress // Int. J. Mol. Sci. 2017. № 18. 30. Mattioli R., Costantino P., Trovato M. Proline accumulation in plants: not only stress // Plant signaling behavior. 2009. Vol. 4, № 11. 31. Moreira A., Moraes L.A.C. Yield, nutritional status and soil fertility cultivated with common bean in response to amino-acids foliar application // Journal of Plant Nutrition. 2017. Vol. 40, № 3. 32. Mousavi S.A., Dalir N., Rahnemaie R. et al. Phosphate and methionine affect cadmium uptake in valerian (Valeriana officinalis L.) // Plant Physiol Biochem. 2021. № 158. 33. Nagata N., Tanaka R., Tanaka A. The major route for chlorophyll synthesis includes (3, 8-divinyl)-chlorophyllide a reduction in Arabidopsis thaliana // Plant and Cell Physiology. 2007. Vol. 48, № 12. 34. Ramyar H., Baradaran-Firouzabadi M., Sobhani A.R. et al. Reduction of lead toxicity effects and enhancing the glutathione reservoir in green beans through spraying sulfur and serine and glutamine amino acids // Environ. Sci. Pollut. Res. 2023. № 3. 35. Ravanel S., Gakière B., Job D. et al. The specific features of methionine biosynthesis and metabolism in plants // Proceedings of the National Academy of Sciences. 1998. Vol. 95, № 13. 36. Sadak M.S., Abd Elhamid E.M., Ahmed M.M.R.M. Glutathione induced antioxidant protection against salinity stress in chickpea (Cicer arietinum L.) plant // Egypt. J. Bot. 2017. Vol. 57, № 2. 37. Santi C., Zamboni A., Varanini Z. et al. Growth stimulatory effects and genome-wide transcriptional changes produced by protein hydrolysates in maize seedlings // Frontiers in Plant Science. 2017. Vol. 8. 38. Teixeira W., Fagan E.B., Soares L.H. et al. Seed and foliar application of amino acids improve variables of nitrogen metabolism and productivity in soybean crop // Frontiers in Plant Science. 2018. Vol. 9. 39. Zou Z., Deng G., Peng X. et al. Effects of exogenous amino acids application on nitrogen accumulation, yield and grain quality in rice // International Journal of Agriculture and Biology. 2018. Vol. 20, № 9.