The role of exogenous influence of caffeic acid on barley plants

Abstract

Currently, the physiological and biochemical role of certain groups of phenols that have high biological activity remains poorly understood. Thus, the functions of hydroxycinnamic acids, related to phenylpropanoids, and, in particular, caffeic acid (CA), remain unclear.

The purpose of this work was to study the effect of different concentrations of caffeic acid on barley productivity, both under normal plant development conditions and in the presence of salt stress.

In a series of laboratory experiments using different test cultures and in a growing season on spring barley plants Hordeum vulgare L., the effect of CA was studied. The activity of different concentrations of caffeic acid was determined using several methodological approaches to phytotesting: the express method in Petri dishes; in plastic flat tablets using the applicate method; in conditions of growing plants in aqueous solutions. The vegetation experiment was carried out on gray forest medium-loamy soil with an organic matter content of 3.8%, a slightly acidic reaction of the soil solution (pH_KCl 5.9). NaCl solution (7.5 g×l^–1) was added to the 2–3 leaf phase. Plants were treated with CA foliarly once during the tillering phase. Three concentrations of CA were used in the experiment, based on the results of preliminary laboratory experiments: 100 µM, 50 µM, 10 µM. At the beginning of booting, the barley plants were harvested and soil samples were taken. Potassium, sodium and total nitrogen content were determined in dry plant mass. The flag leaf of plants was used to determine the content of chlorophyll and carotenoids.

According to the results of phytotesting, it was established that for monocotyledonous and dicotyledonous crops the greatest activity is manifested in variants with the lowest concentrations of CA (10 and 25 μM), while higher concentrations of CA (50 and 100 μM) significantly reduce all test indicators. Based on the results of the growing season, it was established that CA does not have a reliable stimulating effect. Under conditions of sodium chloride salinity with the use of a high concentration of CA (100 μM), a slight increase in the potassium content in the soil occurs. The sodium content in the soil decreased with the use of CA. The total plant weight per vessel decreased significantly from 1.25g/vessel to 0.25g/vessel. Without the use of NaCl, CA treatment was not accompanied by significant changes in barley weight. The content of chlorophyll (a and b) in the control variant (1.83 mg×g^–1) was significantly lower compared to their content in the leaves of plants that were treated with CA (4.03 mg×g^–1 — 10 µM and 3.14 mg×g^–1 — 100 µM). Against the background of NaCl, the content of total chlorophyll decreased to 0.24 mg/g, and when plants were treated with CA it increased from 1.26 mg×g^–1 at low concentration (10 µM) and to 2.14 mg×g^–1 at high concentration (100 µM). The use of CA at a concentration of 100 µM was accompanied by an increase in carotenoid content from 0.22 mg×g^–1 to 0.61 and, against the background of salinity, from 0.16 to 0.38 mg×g^–1. Thus, the use of CA for treating plants during the growing season under salinity conditions affects changes in physiological processes, which contributes to their adaptation.

References

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